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The Human Protein Atlas displays high resolution, multicolor images of proteins labeled with immunofluorescence at the single cell level. This provides spatial information on protein expression patterns to define the subcellular localization to cellular organelles and structures.
Antibodies used for Western blot, immunocytochemistry and immunohistochemistry in the Human Protein Atlas undergo enhanced antibody validation based on the five " pillars" described by the International Working Group for Antibody Validation ( IWGAV), presented in " A proposal for validation of antibodies" ( Uhlen et al ). The enhanced validation principles are adapted for validation in Western.
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